Electroelution (wet, semi-dry, and dry) and diffusion transfer techniques all have their merits and applications, but it is important to note that method alone will not guarantee a successful blot. The protein transfer stage should carry equal risks to PAGE, although many labs adopt the method depicted in of the aforementioned article, which introduces further possibility of electric shock.There are many safer methods for transferring proteins to a membrane. The buffer should be covered at all times when attached to the power supply to prevent contact with the electrified liquid. Some tanks allow the unit to be plugged into the power pack without covering the buffer. Polyacrylamide gel electrophoresis (PAGE) is only unsafe if the equipment is not used correctly. ![]() ![]() To limit risk they should be handled with care, in a fume hood with the use of personal protective equipment.
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